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KMID : 0043319940170010048
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Archives of Pharmacal Research
1994 Volume.17 No. 1 p.48 ~ p.50
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p-Coumaroylamino Acids from Yeast-Elicited Ephedra distachya Cultures
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Song Kyung-Sik
Sankawa Ushio
Ebizuka Yutaka
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Abstract
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Three p-coumaroylamino acids (p-CAAs) were isolated from the yeast-elicited Ephedra distachya cultures by consecutive purification using XAD_2, silicagel and RP-HPLC. Retention times on HPLC as well as their UV, IR, NMR and MS spectral data indicated that the yeast-induced p-CAAs wre p-coumaroyl--D-valine, p-coumaroyl-D-serine and p-coumarouyl-D-threonine, respectively. The structures of p-CAAs were confirmed by the comparison of their physico-chemical properties 3with those of synthetic ones. They were isolated and identified for the first time from natural products and supposed to be accumulated as phytoalexins of Ephedra.
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KEYWORD
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Ephedra distachya, Ephedraceae, Cell suspension cultures, p-Coumaroyl-D-valine, p-coumaroyl-D-serine, p-Coumaroyl-D-threonine, Yeast elicitor
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